Determination of 25-Hydroxyvitamin D in Serum by HPLC and Immunoassay
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چکیده
منابع مشابه
Determination of 25-hydroxyvitamin D in serum by HPLC and immunoassay.
Vitamin D status is usually assessed by measuring the serum concentration of 25-hydroxyvitamin D [25(OH)D]. Its measurement is important as a clinical indicator of nutritional vitamin D deficiency, which is one of the causes of osteoporosis (1). Vitamin D exists in two forms: cholecalciferol (vitamin D 3) and ergocalciferol (vitamin D 2). Vitamin D 2 is further metabolized to 25(OH)D 2. Vitamin...
متن کاملSerum 25-hydroxyvitamin D (25-OH-D) in obese adolescents.
BACKGROUND There is increasing evidence that vitamin D deficiency is common and has been associated with several non-bone related outcomes, including insulin resistance, type 2 diabetes and cardiovascular disease. The influences of gender, puberty, and adiposity on serum hydroxyvitamin D (25-OH-D) levels and the relationship between 25-OH-D and insulin resistance in obese children were studied....
متن کاملInterlaboratory comparison of 25-hydroxyvitamin D determination.
This interlaboratory study on determination of 25-hydroxyvitamin D (25-OH-D) in serum involved 15 laboratories in eight European countries. All could distinguish between normal (50 +/- 31 nmol/L, mean +/- SD) and grossly increased concentrations, but for eight laboratories the results for serum samples with low and normal 25-OH-D content overlapped. In general, values were well reproducible, bu...
متن کاملEnzyme immunoassay for measuring 25-hydroxyvitamin D3 in serum.
We developed a rapid, competitive enzyme immunoassay (EIA) for measuring 25-hydroxyvitamin D3 [25(OH)D3] in serum. The EIA was based upon 25(OH)D3-3-hemisuccinate covalently coupled to secondary amino groups grafted onto the polystyrene surface of microtiter wells. Optimal coupling conditions were established, and we found that inclusion of 40 mumol/L chloramine T, an agent not previously descr...
متن کاملLow Serum Levels of 25-Hydroxyvitamin D in Association with Type 2 Diabetes in Indian Patients
Objective: Vitamin D deficiency is a common disorder worldwide and is indicated by low serum 25-hydroxyvitamin D levels. Recent studies have suggested an association of 25-hydroxyvitamin D deficiency with diabetes mellitus. The objective of this study is to investigate the relationship between 25-hydroxyvitamin D concentration and type 2 diabetes in South Indian patients. Materials and Methods...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 2003
ISSN: 0009-9147,1530-8561
DOI: 10.1373/49.9.1521